The resulting ES cells (clone Ai14) with the PGK-FRT-Neo-poly A cassette deleted (leaving a single att L site) were injected into recipient blastocysts.
High percentage chimeric males were sent to The Jackson Laboratory.
Ai14 mice homozygous for this Rosa-CAG-LSL-td Tomato-WPRE conditional allele (also called Ai14D, Ai14(RCL-td T)-D or Rosa-CAG-LSL-td Tomato-WPRE::ΔNeo) are viable and fertile.
A lox P-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant td Tomato (see below).
As such, it is recommended that researchers include Cre-negative Ai14 controls to establish the baseline td Tomato levels in their experiments.
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