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The resulting ES cells (clone Ai14) with the PGK-FRT-Neo-poly A cassette deleted (leaving a single att L site) were injected into recipient blastocysts.

High percentage chimeric males were sent to The Jackson Laboratory.

Ai14 mice homozygous for this Rosa-CAG-LSL-td Tomato-WPRE conditional allele (also called Ai14D, Ai14(RCL-td T)-D or Rosa-CAG-LSL-td Tomato-WPRE::ΔNeo) are viable and fertile.

A lox P-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant td Tomato (see below).

As such, it is recommended that researchers include Cre-negative Ai14 controls to establish the baseline td Tomato levels in their experiments.

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You're immediately ready to jump in and attempt over 100 free chat line numbers after the minutes are added. Call today and enjoy the most real and also the very best dating encounter in 2016! Your greeting introduces you to other callers, so don't be bashful! Let your voice show off your real self with instant phone chat with other flirty local singles.Experience new exciting connections in the comfort of your home.These Ai14 mice are useful as a Cre reporter strain - expressing td Tomato fluorescence following Cre-mediated recombination.Importantly, the donating investigator reports that very low levels of td Tomato expression may be present prior to introduction of Cre recombinase - but the td Tomato expression levels after Cre recombination are significantly greater than those baseline levels.

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